Detection of AmpC β-lactamase and drug resistance of
Rong WANG, Shangwei WU, Xue LI, Ping HE, Yunde LIU
Frontiers of Medicine
2009,
Volume 3,
Issue 1,
Pages 72-75
doi:
10.1007/s11684-009-0004-y
Abstract:
In order to provide useful information for effective control and clinical therapy of infection, the resistance status and the rate of carryingAmpC β-lactamase of ( ) were investigated. By VITEK (Bacterial automatic biochemical analyzer), the isolates of were identified and the drug resistance was measured. The AmpC enzyme was detected by the five-disk diffusion test.Antibiotic sensitivity test showed that the resistance effects of to cefazolin, cefoxitin and ampicillin were more serious, with resistant rates of 80.5%, 75.3% and 70.1%, respectively. However, it was more sensitive to Sulperazone (cefoperazone/sulbactam, 13.0%), amikacin (16.9%) and ciprofloxacin (19.5%). Meanwhile, the phenotype detection showed that 35.06% (27/77) isolates of produced AmpC β-lactamase. Most of are multi-drug resistant strains. Sulperazone (cefoperazone/sulbactam), a kind of component β-lactamase, is a more effective antibiotic for treating infection caused by . Unreasonable application of the third generation cephalosporins plays an important role in leading to emergence of high-yield AmpC β-lactamase strains, so antibiotics should be used wisely.
Keywords:
Enterobacter cloacae
AmpC β-lactamase
drug resistance